During May-June 2016, little leaf and phyllody signs were noticed on 8% of sesame crop in Chakbhalwal and Kotbhalwal regions of Jammu, India. Phytoplasma suspected witches’ broom symptoms on Cannabis sativa subsp. 5 to 12% in numerous fields. Total genomic DNA from both symptomatic sesame and Cannabis Doctors New York plants have been subjected to nested PCR assays utilizing two units of universal phytoplasma particular primers P1/P7 followed by R16F2n/R16R2 and 3Far/3Rev to amplify the 16S rDNA fragments. First round PCR amplification with primer pair P1/P7 did not yield anticipated 1.8 kb products of 16S rRNA gene region from any of the symptomatic take a look at samples. However, DNA specific fragments of roughly 1.2 and 1.3 kb have been amplified from sesame and C. sativa sub sp. R16F2n/R16R2 and 3Far/3Rev primer pairs, respectively. BLASTn comparability and phylogenetic analysis of 16SrDNA phytoplasma sequences of sesame and cannabis phytoplasma strains revealed the association of ‘C. Phytoplasma asteris’ (16Sr I group) with symptomatic samples. The digital RFLP analysis of the 16Sr DNA sequence of sesame and cannabis additional recognized as phytoplasma strains of 16SrI-B subgroup.
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